Journal: PLoS ONE

Article Title: Effect of TGF-β1 on eosinophils to induce cysteinyl leukotriene E 4 production in aspirin-exacerbated respiratory disease

doi: 10.1371/journal.pone.0256237

Figure Lengend Snippet: Demographic data from the study subjects enrolled in the first cohort of adult asthmatic patients.

Article Snippet: The antibodies used were as follows: TGF-β1 receptor (TGFR1; Abcam, Cambridge, United Kingdom; 1:1,000; 45 kDa), TGF-β2 receptor (TGFR2; Abcam; 1:1,000; 75 kDa), LTC 4 S (Sigma-Aldrich; 1:500; 40 kDa), p38 (Cell Signaling Technology; 1:1000; 38 kDa), phospho-p38 (Cell Signaling Technology; 1: 500; 38 kDa), and actin (Santa Cruz, Dallas, TX, USA; 1:1,000; 42 kDa).

Techniques:

Journal: PLoS ONE

Article Title: Effect of TGF-β1 on eosinophils to induce cysteinyl leukotriene E 4 production in aspirin-exacerbated respiratory disease

doi: 10.1371/journal.pone.0256237

Figure Lengend Snippet: Characteristics of asthmatic patients with high (≥48.1 ng/mL) and low TGF-β1 ( <48.1 ng/mL) levels in the first cohort.

Article Snippet: The antibodies used were as follows: TGF-β1 receptor (TGFR1; Abcam, Cambridge, United Kingdom; 1:1,000; 45 kDa), TGF-β2 receptor (TGFR2; Abcam; 1:1,000; 75 kDa), LTC 4 S (Sigma-Aldrich; 1:500; 40 kDa), p38 (Cell Signaling Technology; 1:1000; 38 kDa), phospho-p38 (Cell Signaling Technology; 1: 500; 38 kDa), and actin (Santa Cruz, Dallas, TX, USA; 1:1,000; 42 kDa).

Techniques:

Journal: PLoS ONE

Article Title: Effect of TGF-β1 on eosinophils to induce cysteinyl leukotriene E 4 production in aspirin-exacerbated respiratory disease

doi: 10.1371/journal.pone.0256237

Figure Lengend Snippet: Demographic data of the study subjects enrolled in the second cohort.

Article Snippet: The antibodies used were as follows: TGF-β1 receptor (TGFR1; Abcam, Cambridge, United Kingdom; 1:1,000; 45 kDa), TGF-β2 receptor (TGFR2; Abcam; 1:1,000; 75 kDa), LTC 4 S (Sigma-Aldrich; 1:500; 40 kDa), p38 (Cell Signaling Technology; 1:1000; 38 kDa), phospho-p38 (Cell Signaling Technology; 1: 500; 38 kDa), and actin (Santa Cruz, Dallas, TX, USA; 1:1,000; 42 kDa).

Techniques:

Journal: PLoS ONE

Article Title: Effect of TGF-β1 on eosinophils to induce cysteinyl leukotriene E 4 production in aspirin-exacerbated respiratory disease

doi: 10.1371/journal.pone.0256237

Figure Lengend Snippet: Effect of TGF-β1 on (A) LTC 4 S expression and (B) LTC 4 S levels in peripheral eosinophils in a time- or dose-dependent manner (samples from 3 asthmatic patients were pooled). (C) Function of dexamethasone against TGF-β1 treatment (samples from 3 asthmatic patients were pooled). (D) Comparison of LTC 4 S levels between ARED and ATA patients (n = 3 asthmatic patients per group).

Article Snippet: The antibodies used were as follows: TGF-β1 receptor (TGFR1; Abcam, Cambridge, United Kingdom; 1:1,000; 45 kDa), TGF-β2 receptor (TGFR2; Abcam; 1:1,000; 75 kDa), LTC 4 S (Sigma-Aldrich; 1:500; 40 kDa), p38 (Cell Signaling Technology; 1:1000; 38 kDa), phospho-p38 (Cell Signaling Technology; 1: 500; 38 kDa), and actin (Santa Cruz, Dallas, TX, USA; 1:1,000; 42 kDa).

Techniques: Expressing

Journal: British Journal of Pharmacology

Article Title: Low‐concentration DMSO accelerates skin wound healing by Akt / mTOR ‐mediated cell proliferation and migration in diabetic mice

doi: 10.1111/bph.15052

Figure Lengend Snippet: DMSO promotes the migration of diabetic mouse‐derived primary keratinocytes in an indirect manner mediated by fibroblast‐derived TGF‐β1. (a) Migration of the keratinocytes by transwell assays after treatment with or without 5‐mM DMSO or TGF‐β1 for 24 h. Data are presented as the mean ± SEM with n = 5 for all groups, analysed by one‐way ANOVA with Kruskal–Wallis test for multiple comparison. Significance level: *P < 0.05, compared with the control group. NS, not significant. (b) Representative H&E‐stained sections of wound tissue 8 days after injury. The arrow indicates the length of the migrating epithelial tongue. Data are presented as the mean ± SEM with n = 6 for all groups, analysed by Student's t test. Significance level: *P < 0.05, 5‐mM DMSO versus the control group. Scale bars represent 100 μm; original magnification ×100. (c) Immunofluorescence staining of TGF‐β1 in DMSO‐treated and untreated wound tissue (n = 6). Scale bars represent 50 μm; original magnification ×200. (d) Migration of keratinocytes treated as indicated. MF, cocultured with mouse fibroblasts by a transwell assay; 1D11, a TGF‐β1 neutralizing antibody. Data are presented as the mean ± SEM with n = 5 for all groups, analysed by one‐way ANOVA with Kruskal–Wallis test for multiple comparison. Significance level: *P < 0.05, compared with the MF group, (e) western blot of migration‐related proteins in the keratinocytes treated as indicated

Article Snippet: According to the stimulants added to the medium in the bottom chamber, six experimental groups were established: PBS control, DMSO (5 mM), recombinant mouse TGF‐β1 (5 ng·ml −1 , R&D Systems, Minnesota, USA), mouse fibroblasts (MFs, 4 × 10 5 cells), MF + DMSO and MF + DMSO+1D11 (a TGF‐β1 (TGFBR1) neutralizing antibody, 10 g·ml −1 , R&D Systems, Minnesota, USA).

Techniques: Migration, Derivative Assay, Staining, Immunofluorescence, Transwell Assay, Western Blot

Journal: Biology of reproduction

Article Title: Transforming growth factor beta1 from endometriomas promotes fibrosis in surrounding ovarian tissues via Smad2/3 signaling.

doi: 10.1093/biolre/iox140

Figure Lengend Snippet: Figure 6. Analyzing the fibrotic effects of TGF-β1 on EdSCs in vitro. After EdSCs were exposed with TGF-β1, fibrosis-related genes including COL1A1, ACTA2, CTGF, and FN1 at 4, 12, 24, 48, and 72 h were detected by RT-qPCR (A), and fibrosis-related markers including COL1A1, ACTA2, CTGF, and MMP2 were analyzed by western blotting (B). The components’ expression of the TGF-β1/Smad signaling pathway such as SMAD2, p-SMAD2, SMAD3, and p-SMAD3 were demonstrated by western blotting analysis (C).

Article Snippet: Briefly, a series of 5-μm sections was incubated with the following antibodies: monoclonal rabbit antibodies against human TGFB1 (1:200), TGF-β1 receptor (TGFBR1; 1:500; sc-398; Santa Cruz Biotechnology), SMAD2 (1:200), SMAD3 (1:200), SMAD4 (1:200), SMAD2/3 (1:200), phospho-SMAD2 (p-SMAD2; 1:200), phospho-SMAD3 (p-SMAD3; 1:200), Collagen I (COL1A1, 1:200), connective tissue growth factor (CTGF, 1:1000), alpha-smooth muscle actin (ACTA2, 1:200), cluster of differentiation 10 (CD10, 1:200; ab126593; Abcam), and a monoclonal mouse antibody against human pan cytokeratin (KRT (pan); 1:200; ab7753; Abcam).

Techniques: In Vitro, Quantitative RT-PCR, Western Blot, Expressing

Journal: Biology of reproduction

Article Title: Transforming growth factor beta1 from endometriomas promotes fibrosis in surrounding ovarian tissues via Smad2/3 signaling.

doi: 10.1093/biolre/iox140

Figure Lengend Snippet: Figure 7. IHC detection of activated TGF-β1/Smad signaling in the cystic walls of endometriomas. (A) Representative photomicrograph of an excised endometri- oma stained with Masson’s trichrome after laparoscopic cystectomy. The red lines separated ovarian tissue and the cystic wall, while yellow lines separated the endometriotic loci and the cystic wall. Follicles were highlighted with arrows in (A) and identify ovarian tissue. The expression of TGFB1 (B) and TGFBR1 (C), and fibrosis-related factors including ACTA2 (D), COL1A1 (E), and CTGF (F), and components of the TGF-β1/Smad signaling pathway including p-SMAD2 (G), p-SMAD3 (H), SMAD2/3 (I) SMAD2 (J), SMAD3 (K), and SMAD4 (L) were demonstrated in the serial sections. (M) A comparison among the percentage of positive expression areas of different primary antibody in the cystic walls of endometriomas. Magnified photomicrographs with high-resolution view areas of the blue-square frames were shown in Supplemental Figure S4 correspondingly. (A–L: 100×).

Article Snippet: Briefly, a series of 5-μm sections was incubated with the following antibodies: monoclonal rabbit antibodies against human TGFB1 (1:200), TGF-β1 receptor (TGFBR1; 1:500; sc-398; Santa Cruz Biotechnology), SMAD2 (1:200), SMAD3 (1:200), SMAD4 (1:200), SMAD2/3 (1:200), phospho-SMAD2 (p-SMAD2; 1:200), phospho-SMAD3 (p-SMAD3; 1:200), Collagen I (COL1A1, 1:200), connective tissue growth factor (CTGF, 1:1000), alpha-smooth muscle actin (ACTA2, 1:200), cluster of differentiation 10 (CD10, 1:200; ab126593; Abcam), and a monoclonal mouse antibody against human pan cytokeratin (KRT (pan); 1:200; ab7753; Abcam).

Techniques: Staining, Expressing, Comparison